Advanced Biochemistry

Paper Code: 
25DZOL712
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
1. Enlighten the students about the importance of biological macromolecules, structures and configurational properties.
2. Examine the key role of biochemistry in the metabolic process.
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

25DZOL

702

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Advanced Biochemistry

THEORY

CO:199 Analyze the concepts                                               of biocatalysts, enzyme

activity, its kinetics, inhibition and redox reactions.

CO:200 Acquire detailed knowledge about the structure, classification,

physico-chemical properties and biological significance, metabolism and metabolic disorders of carbohydrates.

CO:201Illustrate the lipid classification, structure, properties, anabolic and catabolic pathways of lipid metabolism with rate-limiting steps and disorders associated with them.

CO:202 Classify proteins and amino acids based on various parameters. Compare and contrast between different levels of protein organization and correlate the protein sequencing strategies with its structures.

CO:203 Outline various catabolic reactions associated with amino acid degradation and correlate the inborn metabolic disorders with their defective metabolic enzymes and their consequences.

CO:204 Contribute effectively in course-specific interaction

Approach      in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration Learning activities                     for the students: Self-learning assignments, Simulation, Seminar presentation, Giving tasks

Class test Semester end examinations, Quiz, Assignments, Presentation Individual                  and group projects

 

12.00
Unit I: 
Enzymes
Biocatalysis
Classification and nomenclature of enzymes
Mechanism of enzyme action
Enzyme Kinetics
Inhibition of enzyme
Isoenzymes and coenzymes
Energy consideration
Biological oxidation and reduction
Fundamental reactions of biological oxidation
Redox potential and Electron transfer system enzymes
 
12.00
Unit II: 
Carbohydrates
Definition, general properties, classification and configuration of carbohydrates
Metabolism
Catabolism of glucose with Stoichiometry, Glycolysis, Krebs cycle and Cori’s cycle
Phosphogluconate pathways (Pentose Phosphate Pathway) Glycogenesis, Glycogenolysis and Gluconeogenesis
Inborn errors of metabolism: Galactosemia, Lactose Intolerance, Glycogen storage diseases.
 
12.00
Unit III: 
Lipids
Definition, general properties and classification Structure and importance of-:
Simple lipids- fats and wax
Compound lipids- phospholipids, glycolipids, amino lipids and sulpholipids Steroids- cholesterol, bile acids, steroid hormones Lipoproteins
Lipid Metabolism
Beta oxidation and Lipogenesis.Inborn errors of metabolism: Niemann pick disease,TaySach’sdisease,Gaucher’s disease, Krabbe’s disease
 
12.00
Unit IV: 
Proteins
General properties and classification of proteins
Amino acid structure and classification
Structure of protein-Primary, Secondary, Tertiary And Quaternary
Ramachandran plots
Globular and fibrous protein- motif in globular proteins
Protein Sequencing
 
12.00
Unit V: 
Protein metabolism
Amino acid degradation: Deamination reaction- Oxidative and non oxidative deamination reaction, Transamination and Trans deamination, Decarboxylation, Ornithine cycle of urea formation, Fate of carbon skeleton of amino acids(Metabolism of individual amino acid excluded)
Inborn errors of metabolism: Alkaptonuria, Phenylketonuria, Maple Syrup, Tyrosinemia, Albinism, Urea Disorders.
 
Essential Readings: 
● U. Satyanaryana . Biochemistry IV Edition, Elsevier (2013)
● Jain,J.L. Fundamentals of Biochemistry ,S.Chand publishers New Delhi.
● Medical Physiology; Chatterjee, M.N and Shinde, R.; Jaypee brothers.(2007)
● Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006). Biochemistry. VI Edition. W.H Freeman and Co.
● Lehninger, A.D. Principles of Biochemistry, CBS Publishers and Distributors, Shahdara, Delhi.
● Nelson, D. L., Cox, M. M. and Lehninger, A.L. (2009). Principles of Biochemistry. IV Edition. W.H. Freeman and Co
 
SUGGESTED READINGS:
● Harvey, Richard A., Ph. D. (2011). Lippincott's illustrated reviews: Biochemistry. Philadelphia :Wolters Kluwer Health
● Tortora, G.J. and Derrickson, B.H. (2009). Principles of Anatomy and Physiology, XII Edition, John Wiley & Sons, Inc. 
● Principles of Biochemistry; Smith et.al; McGraw Hill, New York.
● Textbook of Medical Biochemistry; Ramakrishnan, S. Prasanan, K.G and Rajan, R; Orient Longman, Madras
● Conn, E.E. and Stuff, P.K. Outlines of Biochemistry,John Wiley New York.
● Donald Voet and Judith G. Voet Biochemistry, John Wiley and Sons., New York.
● H.S. Srivastava, Element of Biochemistry, Rastogi Publications Meerut.
 
References: 
E-RESOURCES:
● e-PGPATHSHALA-INFLIBNET CENTRE
● Biochemistry- Swayam Prabha-https://dth.ac.in/medical/course.php
 
e-JOURNAL:
● Journal of Biochemistry- ISSN 1756-2651
● Biochemistry- ACS Publication- ISSN 1520-4995
● Journal of Cellular Biochemistry- Wiley online Library- Online ISSN: 1097-4644 
 

 

Academic Year: 
2025-2026
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