CELL AND MOLECULAR BIOLOGY II (Practical)

CELL AND MOLECULAR BIOLOGY II (Practical) [1]

Paper Code:

24ZOL424B

Objective:

Course objectives:

This course will enable the students to –

1. Develop a hands on understanding of Immunoassays, precipitation techniques and immunodiffusion methods

2. Appraise histological and histochemical techniques

3. Differentiate and evaluate detailed practical knowledge of different biochemical methods

Course Outcomes:

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessme nt Strategies

Course Code

Course Title

24ZOL

424B

Cell and Molecular Biology II (Practical)

The students will

CO193. Evaluate and perform different biochemical methods, Immunoassays and quantitation of antibodies

CO194. Analyse and prepare histological slides by microtomy and histochemical techniques

CO195. Design and understand various

immunohistochemistry protocols CO196. Differentiate and evaluate detailed practical knowledge of different biochemical methods and Make use of permanent slide to study different cell types

CO197. Reflect on personal learning experiences and experimental outcomes during viva voce assessments

CO198. Contribute effectively in course-specific interaction

Approach in teaching: Practical, Reading assignments, Demonstration

Learning activities for the students: Practical knowledge, Effective questions, Seminar presentation

Class test Semester end examinations Viva voce

● Quantitation of antibodies-

● Precipitation Techniques

● Immunodiffusion diffusion method.

● Preparation of biological tissues and sectioning for paraffin wax histology by microtomy

● Histochemistry

● Carbohydrate- PAS method

● Proteins- Ninhydrin Method

● Nucleic acid- Feulgen Method

● Localization of acid & alkaline phosphatase Ascorbic Acid

● Determination of optimum concentration of enzyme for kinetic studies

● Estimation of acid & alkaline phosphatase by biochemical method

● Immunoassays-ELISA

● Permanent slides (for Spotting)- Blood cells, Thymus, Spleen, Liver

Essential Readings:

● Shelve P. and Blanch DEW, Cell and Molecular Biology. John Wile) and Sons Inc., New York, 1994.

● Darnell, J.L. Lodish, H and Baltimore, D. Molecular Cell Biology, Scien tific American Books Inc. New York.

● Watson, J.D., Hopkins, N.H. Roberts, J.W Steitz, J.A. and Weiner A.M. Molecular Biology of the Gene. The Benjamin/Cumings Pub. Co. Inc. California

● Gardner, EJ. Simons, MJ. and Snustad, D.P. Principles of Genetics. John Wiley .& Sons Inc. New York

● Voet, D. and Voet, J.G. Biochemistry, John Wiley & Sons Inc New York.

● Zubay, G.L. Parson, WW and Vance, D.E. Principles of Biochemistry. Wm.C. Brown Publishers, Oxford, England.

● Goldsby, RA.. Kindt, T.J. and Osborne, B.A. Kuby Immunology, WH Free- I man & Co. New York.

References:

SUGGESTED READINGS:

● Old R. W. and Primrose S. B. Principles of Gene Manipulation An introduction to genetic engineering. Blackwell Scientific Pub li- cation. London, 1989.

● Twyman R. M. and Wisden W. Advanced Molecular Biology A Concise Reference Viva Books Pvt. Ltd. New Delhi: 1999

● Gasque, C. E. A. Manual of laboratory experiences in Cell Biology Universal Book Stall New Delhi.

● Meyers R.A. Molecular Biology and Biotechnology A comprehen- sive desk Reference. VCH Publishers, Inc. New York, 1995

E- RESOURCES

chromeextension://efaidnbmnnnibpcajpcglclefindmkaj/https://faculty.ksu.edu.sa/sit [2] es/default/files/bch361_handnote_1.pdf

JOURNALS:

https://www.cellmolbiol.org/ [3] https://academic.oup.com/jmcb [4]

Academic Year:

2024-2025 [5]

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